[PDF] Verification of the Abbott Alinity m Resp-4-Plex assay for detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus | Semantic Scholar (2024)

The results show that the multiplex molecular approach is a valuable tool for the simultaneous laboratory diagnosis of SARS-CoV-2, Flu-A/B, and HRSV in hospitalized and outpatients.

The clinical presentation of Influenza and SARS-CoV-2 and techniques available for diagnosis are discussed, and available data regarding the multiplex diagnostic assay of both viral infections are summarized.

Since RSV vaccinations are still under investigation, administration of palivizumab should be considered in high-risk patients and greater attention should be paid to a further resurgence of RSV in the post-COVID-19 era.

The ability to assess infectiousness of samples previously frozen in transport medium appears to be maintained, as the effect of freeze-thaw on viral culture isolation from a large number of clinical samples was examined.

Current use case scenarios for SARS-CoV-2 antigen, nucleic acid amplification, and immunologic tests are considered, incorporating the latest evidence for analytical/clinical performance characteristics and advantages/limitations for each test type to inform current debates about how tests should or should not be used.

The frequency of influenza-A and B antigens in swab samples is determined and their potential changes at the time of initial diagnosis in differentiating the diagnosis of influenza infection from other causes of upper respiratory tract infection by physical examination and clinical vital signs in the emergency room is examined.

Two automats are compared: Alinity ci ® and Architect ci-8200® Abbott, to show the results of the protocol of verification of the method of determination of aspartate aminotransferase (AST).

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The assay LoD meaningfully impacts clinical performance of SARS-CoV-2 tests and assays should be benchmarked against a universal standard to allow cross-comparison of Sars-Cov-2 detection methods.

The analytical performance of the Abbott RealTime SARS-CoV-2 assay on the m2000 system was verified and its clinical performance was compared to the CDC 2019-nCoV real-time PCR diagnostic panel and the Thermo Fisher TaqPath RT-PCR COVID-19 kit.

The author addresses several areas of current debate, including the role of molecular assays in defining the duration of isolation/quarantine, whether the PCR cycle threshold value should be included on patient reports, and whether testing a large percentage of the population is feasible and represents a viable strategy to end the pandemic.

The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for SARS-CoV-2 RT-PCR testing, and in response to this ongoing shortage, in-house production of VTM was began in support of diagnostic testing in the hospital network.

The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCRs) testing, and in response to this ongoing shortage, in-house production of VTM was began in support of diagnostic testing in the hospital network.

It is found that substantial outbreaks of RSV may occur in future years, with peak outbreaks likely occurring in the winter of 2021–2022, and future outbreaks of influenza broadly echo this picture, but are more uncertain.

A Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 genes is developed, providing a rapid and reliable diagnostic tool for HPV detection and typing that is amenable to automation.

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